Brazil scientists test frozen jaguar semen to help species

Brazilian and American scientists have performed artificial insemination using frozen sperm for the first time on a wild-born female jaguar

By DIANE JEANTET and TATIANA POLLASTRI Associated Press

October 30, 2021, 9:30 AM

• 4 min read

JUNDIAI, Brazil — Brazilian and American scientists tranquilized a wild-born female jaguar now living in a protected area in Sao Paulo state. They’re hoping the 110-pound feline named Bianca could make history for the second time in two years.

In 2019, Bianca gave birth to the first jaguar cub ever born from artificial insemination. Now, the 8-year-old could once again advance the cause of preserving her species. That is if all goes according to plan and she becomes pregnant using semen that is frozen.

Scientists say frozen semen would be easy to transport and help ensure the genetic diversity of jaguars whose populations are increasingly fragmented by habitat destruction, according to Lindsey Vansandt, a theriogenologist — a specialist in veterinary reproductive medicine — at the Cincinnati Zoo & Botanical Garden.

“The population sort of becomes smaller and smaller, and then you get inbreeding which has lots of bad consequences,” Vanzandt told The Associated Press moments after performing the procedure on an unconscious Bianca atop a surgery table.

“If we can take sperm from one male and inseminate a female from another location, we can keep their gene flow moving and keep the population more healthy,” Vanzandt said.

Wildlife experts from the Cincinnati Zoo, the Federal University of Mato Grosso, and the environmental organization Mata Ciliar have for years developed their insemination program for the Western Hemisphere’s largest feline. They work with individuals rescued from habitat loss in the Amazon rainforest, Cerrado savanna, and Pantanal wetlands, all of which have suffered a surge of deforestation and fires in recent years.

Some jaguars badly injured by blazes in the Pantanal last year required transport to specialized facilities for care. Others either died or were displaced.

“Look what happened in the Pantanal, the Cerrado,” said Cristina Adania, a veterinarian and coordinator of Mata Ciliar. ““They are being killed before we even get to treat them, so something has to be done.”

This year, a study published by wild cat conservation group Panthera, the Federal University of Mato Grosso do Sul, and partners estimated that almost 1,500 jaguars were killed or displaced by the fire and habitat loss in Brazil’s Amazon from 2016 to 2019.

According to Panthera, displaced jaguars are unlikely to thrive in new environments, which may be the range of another territorial individual. Plus, they are unfamiliar with where best to find prey, leaving them hunting livestock and putting them in ranchers’ crosshairs.

The International Union for the Conservation of Nature’s Red List classifies jaguars as “near threatened” — a grade above vulnerable — though their population is on the decline and their habitat “severely fragmented.”

Bianca was still a cub in the Amazon when she was rescued and delivered to Mata Ciliar. Like some wild-born cats living at the Brazilian Center for the Conservation of Neotropical Felines in Jundiai, she can’t be reintroduced to the wild, Adania said. Another female jaguar at the facility, named Tabatinga, was also artificially inseminated on Thursday.

Unfrozen jaguar semen only stays suitable for a few hours, Vanzandt said. Frozen semen can be used for years but typically has a lower success rate for felines than humans.

If Bianca’s case is successful, it will remove the strain and stress of transporting carnivores that weigh as much as 300 pounds to mate in person. Even when a jaguar is charmed, there’s no guarantee it will get along with its would-be mate, said Adania.

“This is good for genetic diversity, but also towards that larger goal of increasing the number of jaguars,” said Vanzandt. “The dream is to increase the numbers to a stable population.”

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